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3 parts come in a colour no other set has for that mould

• Train FrontYellow · ×2
• Vehicle Base 4 x 10 x 2/3 with 4 x 2 Recessed Center with Smooth UndersideBright Light Orange · ×2
• Hat, Forestmen (Reissue with Blind Hole for Feather)Light Bluish Gray

10 parts appear in only a few other sets

• Tail Shuttle with Gold '60409', Fire Fighter Symbol PrintRedalso in 77944 Fire Station Headquarters (all City)
• Aircraft Fuselage Curved Forward 6 x 8 BottomYellow · ×2also in 76080 Ayesha's Revenge, 60164 Sea Rescue Plane
• Equipment Cup / Mug with Marshmallow with Flames/Fire PrintWhitealso in 60231 Fire Chief Response Truck, 41690 LEGO Friends Advent Calendar
• Hair Short Wavy with Side PartOrangealso in 70615 Fire Mech, 60295 Stunt Show Arena
• Plate 2 x 12Bright Light Yellowalso in 60308 Seaside Police and Fire Mission, 60243 Police Helicopter Chase (all City)
• Brick Round 1 x 1 with 'VITA RUSH' Logo with Green Leaves PrintWhite · ×2also in 60290 Skate Park, 60294 Stunt Show Truck, 40533 Cosmic Cardboard Adventures
• Brick 1 x 16Dark Azurealso in 31077 Modular Sweet Surprises, 31096 Twin-Rotor Helicopter, 11009 Bricks and Lights
• Hair 2 PonytailsDark Azurealso in 60262 Passenger Airplane, 60341 The Knockdown Stunt Challenge, 31141 Main Street
• Hair Elaborate Knot BunBlackalso in 75226 Inferno Squad Battle Pack, 75347 TIE Bomber, 910059 Privateer Frigate Fortuna
• Plate Round Corners 6 x 6 x 2/3 Circle with Reduced KnobsReddish Brownalso in 71368 Toad's Treasure Hunt, 10373 Mini Bonsai Trees, 11512 Hanging Golden Pothos

3 moulds first came out in 2020, including in this set

• Fairing, Motorcycle Frame, Vintage no Front Mudguard
• Plate Round Corners 6 x 6 x 2/3 Circle with Reduced Knobs
• Wheel 14.4 mm with Bar

8 existing moulds first came out in their colour in 2020, including in this set

• Panel 1 x 2 x 1 with Rounded Corners and 2 SidesReddish Brown · ×2
• Plate Round Half 3 x 6 with 1 x 2 CutoutGreen · ×2
• Brick Round 4 x 4 Centre HoleTan
• Hair 2 PonytailsDark Azure
• Hair 2 PonytailsDark Brown
• and 3 more

5 minifigure parts with their print are only in this set

• Costume Corn Cob Suit with Dark Green Leaves PrintDark Green
• Minifig Head Freya McCloud, Green Glasses, Lopsided Grin / Brown Smudge/Mud on…Yellow
• Minifig Head Mayor Fleck, Glasses, Dark Tan Eyebrows, Neutral PrintYellow
• Torso Jacket with Gold Sash with '#1 MAYOR' Print, Dark Green Arms, Yellow HandsDark Green
• Torso, Dual Molded Arms, Muscles, Harness, Gold Police Badge Print, Dark Blue…Dark Blue